Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES HEPES BIOPERFORMANCE 99.5
NC3239327 HEPES BIOPERFORMANCE 99.5
Boston Bioproducts Inc HEPES Buffer, 1 M, pH 7.5, 500 mL
This product is a 1 M HEPES Buffer solution, pH 7.5, which is essential for maintaining stable physiological pH in various biological applications. It is rigorously quality-controlled and specifically formulated for precision in sensitive biochemical and molecular biology research. The high-purity zwitterionic buffering agent is ready-to-use and suitable for diverse applications such as cell culture, protein purification, and live cell imaging.
- Stable pH control maintaining 6.8 to 8.2
- Broad applicability in various research areas
- Convenient format as a ready-to-use solution
- High purity, formulated with USP Compendial-Grade raw materials
- CO₂-independent buffering for reliable pH stability
- Filtered through a 0.22-micron filter for enhanced purity
![Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-D22150-1000.0.JPG-250.jpg)
Research Products International Corp DPBS 1X Solution [Dulbecco's Phosphate Buffered Saline 1X Sterile Solution], 1 Liter
Ready to use sterile 1x solution
Contains: 137 mM Sodium Chloride
2.7 mM Potassium Chloride
10 mM Phosphates
Does not contain Calcium or Magnesium
Research Products International Corp MES, Anhydrous, 100 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
![Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-E58100-500.0.JPG-250.jpg)
Research Products International Corp Ethylenediaminetetraacetic acid [EDTA], 500 Grams
Substance Name: EDTA
CAS Number: 60-00-4
Molecular Formula: C10H16N2O8
Molecular Weight: 292.24
Chemical Purity: >99%
Soluble in: Water
Store at: Room Temperature
Bioworld TAE Buffer (50X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 50X concentration and is filtered through a 0.22 µm membrane. It has a pH of 8.5 ± 0.1. A concentration of 1X contains 0.04M Tris, 0.02M acetate, and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Zymo Research Corporation DNA Elution Buffer, 50ml
This can be used with any of the DNA purification for DNA elution from columns, plates, and magbeads.
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer working solution, MFCD00236358
Tris-Borate-EDTA buffer working solution
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g