Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC 4-morpholineethanesulfonic acid, sodium salt (1:1) | 71119-23-8 | 98.0% | 10 G
MES sodium salt (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer with a buffering capacity ranging from pH 5.5-7.0. It is classified as a Good's buffer and is widely employed in biochemistry and molecular biology experiments, including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer with buffer capacity ranging from pH 5.5-7.0.
- Good's buffer.
- Widely used in biochemistry and molecular biology experiments.
- Provides effective buffering capacity around pH 6.15.
- pKa value changes with temperature, which should be considered for experiments at different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
Medchemexpress LLC MES sodium salt | 71119-23-8 | 98.0% | 50 G
MES sodium salt | 71119-23-8 | 98.0% | 50 G
Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 500 mL
Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659869.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 5 Kg
MES Sodium Salt [2-(N-Morpholino)ethanesulfonic acid] Sodium Salt, 5 Kg, is a widely used buffering reagent known for its ability to stabilize pH in a variety of biological and biochemical applications. With a high buffering capacity, MES is effective in maintaining pH in the range of 5.5 to 6.7, making it ideal for enzyme reactions, electrophoresis, and other experiments requiring precise pH control. The 5 kg weight offers a cost-effective solution for large-scale operations or frequent use. MES is often used in protein chemistry, molecular biology, and cell culture media to ensure consistent conditions during experimental procedures.
Teknova 2M MOPS Buffer, pH 7.4. 500mL, Sterile 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Medchemexpress LLC 4-Morpholineethanesulfonic acid, sodium salt (2:1) | 117961-21-4 | 98.0% | 25 G
MES (2-Morpholinoethanesulphonic acid) hemisodium is an amphoteric ion buffer with a buffer capacity ranging from pH 5.5 to 7.0. It is a Good's buffer widely used in biochemistry and molecular biology experiments.
- Amphoteric ion buffer.
- Buffer capacity ranging pH 5.5-7.0.
- Widely used in biochemistry and molecular biology experiments, such as cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Zwitterionic buffer, providing effective buffering capacity around pH 6.15.
- pKa value changes with temperature, requiring consideration for different temperatures.
- Shows good buffering properties in biological experiments.
- Exhibits poor affinity with metal ions (binding constants at 20 °C and 0.1 M: Mg2+, 0.8; Ca2+, 0.8; Mn2+, 1.7), making it suitable for studies involving metal ions.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications. The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :Useful pH range :6.4 - 7.8pKa (25 °C) :7.1 (Free acid)Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
Teknova MES Dilution Buffer, pH 6.5. 1000mL, Sterile 8 pack.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Medchemexpress LLC 1-Propanesulfonic acid, 3-(bis(2-hydroxyethyl)amino)-2-hydroxy- | 68399-80-4 | MFCD00038353 | 98.0% | 100 G
DIPSO is a biological zwitterionic buffer with a useful pH range of 7.0 to 8.2. It can interfere with meiotic regulation in mouse oocytes and exhibits surfactant activity at 10 mM. This product is for research use only.
- Useful pH range of 7.0 to 8.2
- Can interfere with meiotic regulation in mouse oocytes
- Exhibits surfactant activity at 10 mM
Thermo Scientific Production Chemicals and Services TRIS BIOTECH USP GMP 10KG
NC3849494 TRIS BIOTECH USP GMP 10KG
![eMolecules 1087784-78-8 | tert-butyl 1-(hydroxymethyl)-3-azabicyclo[3.1.1]heptane-3-carboxylate | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502136115.JPG-250.jpg)
eMolecules 1087784-78-8 | tert-butyl 1-(hydroxymethyl)-3-azabicyclo[3.1.1]heptane-3-carboxylate | 1g
ChemScene | 6-Bromo-8-methylimidazo[12-a]pyrazine | 100mg | 632289319 | CS-0152803 | 1159815-50-5 | MFCD12033249 | 212.050 | C7H6BrN3
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